Surface-Enhanced Raman Spectroscopy (SERS) for characterization SARS-CoV-2

We used SERS with silver nanoparticles (AgNPs) as the active substrate to develop a, simple, quick, and accurate method for detection characterization SARS-CoV-2 without need RNA isolation purification. Inactivated was used. The signals were more than 105 times enhanced normal Raman (NR) spectra. spectra of fingerprint revealed pronounced intensity nucleic acids; aromatic amino acid side chains: 1007 cm−1 (Phe marker), 1095 (CN PO2− markers), 1580 (Tyr, Trp markers). Vibrations protein main chain: 1144 NH2 1221 NH 1270 (NH2 1453 (CHCH2 marker). All these biomolecules could be adsorbed on AgNPs surface's dense hot patches. band varied concentration SARS-CoV-2, a virus limit less 103 vp/mL RSDs 20 %.